The Effect of Magnetite Nanoparticles on the Growth and Development of Nicotiana Tabacum Plants in Vivo and in Vitro Culture

Background. Nanomaterials are easily modified and have unique characteristics associated with a large reactive surface Due to these properties, nanomaterials used in various branches of sciences technology, such as pharmaceuticals, biotechnology, chemical etc. Recently, the effect magnetite nanoparticles on morphological properties plants has been actively studied for their further use nanoadditives increase yields improve agricultural plants. Tobacco (Nicotiana tabacum) is model object plant it study factors dicotyledonous plants, so was chosen growth, development, mass accumulation by Objective. We aimed growth development Nicotiana tabacum vivo vitro. Methods. The ability tobacco produce biogenic magnetic searching mammal proteins homologues theNicotiana proteome using Blast NCBI program comparative genomics methods. were divided into groups (control, nanoparticle concentration 0.1 mg/cm3, 1 mg/cm3) both vitro experiments. Analysis parameters performed every 14 days dynamics effects nanoparticles. Results. It determined that at mg/cm3 culture significantly affect root system sprouts tabacum. On 56th day cultivation salivary medium supplemented an shoot length 13.3%, 31.7%, absolutely dry substances 18.75% observed compared control. Treatment suspension led more pronounced results when growing vivo. So, the56th day, increased 23.3%, – 19.2%, by2 times, first leaves appeared 2 earlier addition substrate which grown inhibits tobacco. Conclusions. Studies shown expediency nanofertilizers cultivation.